[ effect of antioxidant compounds and media on biosynthesis of limiter phenolic compounds during in vitro culture of Mentha arvensis L.]

Background: Phenolic compounds are one of the most important classes of plant secondary metabolites that produced in response to environmental conditions. The concentrations and compounds of media have played a significant role in production of these compounds. Production of phenolic compounds in the medium reduces the chances of success in tissue culture process or complicates the process

Objective: This study aimed to investigate the effect of various antioxidants and media on change in biosynthesis of phenolic compounds in callus culture of Mentha arvensis L

Methods: leaves were cultured on MS medium supplemented with 2,4-D, NAA, BAP and KIN for callus induction. After selection of the best medium for callus induction, calli were subculture on MS and B5 media in combination with different concentration of Activated charcoal, Citric acid, Ascorbic acid, Turmeric and polyvinylpyrrolidone [PVP] for study of phenolic compounds. The amounts of phenolic compounds in callus and media were analyzed by spectrophotometer

Results: The maximum callus fresh weight [3.56 gr] and dry weight [1.88 gr] were obtained in 0.5 mg/l 2,4-D. Activitated charcoal, citric acid, Ascorbic acid, PVP, have not significant effects on production of phenolic compounds whereas, both concentrations of Turmeric have significant effect. The highest level of phenol [127 ml/micro g] in media and the lowest level [72.9 ml/micro g] of phenol in callus was observed in media with 1000 mg/l of Turmeric

Conclusion: Turmeric will be assisting a significant effect on release and absorption of phenolic compounds in tissue culture medium. This study could be contributed as an inexpensive and practical for callus induction and also could applied for production of phenolic compounds in vitro plant secondary metabolites production

Cytotoxicity and antioxidant activity of five plant species of solanaceae family from Iran

The use of natural products as anticancer and antioxidant agents has a long history. Several drugs currently used in chemotherapy were isolated from plant species. The aim of this study was to evaluate cytotoxicity and antioxidant activity as well as phenol and flavonoid contents of five plant species of Solanaceae family. Five plant species of Solanaceae family were collected from different regions of Iran. Methanol extracts and chloroform fractions of these species were tested by brine shrimp lethality assay in order to detect cytotoxicity. Antioxidant activity was evaluated by DPPH method. The total phenol content was measured using Folin - Ciocalteu method. The flavonoid content was measured by a colorimetric assay. The extracts of Datura innoxia and Datura stramonium showed the highest cytotoxicity activities with LC[50] values of 22.08 and 21.66 micro g/ml, respectively. The chloroform fractions of these two species were subjected to cytotoxicity assay with LC[50] values of 33.00 and 4.29 micro g/ml, respectively. In comparing, Solanum dulcamara showed the highest antioxidant activity with IC[50] values of 52.51 micro g/ml and the highest phenol and flavonoid content of the dry weight. It could be seen among five tested plant species that D. stramonium had the highest cytotoxic activity and S. dulcamara had the highest antioxidant activity, phenol and flavonoid content. Further studies are necessary for chemical composition of the extracts and more comprehensive biological assays